The 48-year-old rap star has ridiculed Kanye for his support of US President Donald Trump and for wearing his 'Make America Great Again' cap in his verse on 'What's Free', a new song that features on Meek Mill's album 'Championships'.
Jay - who is married to pop star Beyonce - raps: "No red hat, don't Michael and Prince me and Ye / They separate you when you got Michael and Prince's DNA, uh / I ain't one of these house n***** you bought / My house like a resort, my house bigger than yours."
Then, Jay - who has kids Blue Ivy, six, and 17-month-old twins Rumi and Sir with Beyonce - seemingly compared his wife to Kanye's spouse, reality TV star Kim Kardashian West.
He raps: "My spou... (C'mon man). My route better, of course."