"It's a humbling thing when you find someone to love," Clooney said, looking out at her. "Even better when you've been waiting your whole life.
"He recalled how actors who are nominated but don't win awards can go home feeling like losers and reminded those in the audience that they aren't.
"If you are in this room, you've caught the brass ring," he said. "You've gotten to do what you've always dreamed of doing. That isn't losing."
Clooney wore a "Je Suis Charlie" button on his tuxedo lapel in support of the French satirical magazine that was targeted by gunmen in France last week. He saluted the millions of people who honoured the victims by marching in the street this weekend.
"They didn't march in protest," Clooney said. "They marched in support of the idea that we will not walk in fear."
Fargo and Transparent have been the standout winners so far at today's Golden Globe awards.
Lorde missed out on her chance at a trophy, with Best Original Song going to John Legend's Glory from the film Selma.
Kevin Spacey won his first award - after eight nominations - for his role as Frank Underwood in House of Cards, while George Clooney's lifetime achievements were celebrated with the Cecil B. DeMille Award.
Here's a full list of the winners - and for a recap of the action, see our live blog below.
MOTION PICTURES
• Picture, Drama: "Boyhood."
• Picture, Musical or Comedy: "The Grand Budapest Hotel."
• Actor, Drama: Eddie Redmayne, "The Theory of Everything"
• Actress, Drama: Julianne Moore, "Still Alice."-Director: Richard Linklater, "Boyhood.
• Actor, Musical or Comedy: Michael Keaton, "Birdman."
• Actress, Musical or Comedy: Amy Adams, "Big Eyes."
• Supporting Actor: J.K. Simmons, "Whiplash."
• Supporting Actress: Patricia Arquette, "Boyhood."
• Foreign Language: "Leviathan."
• Animated Film: "How to Train Your Dragon 2."
• Screenplay: Alejandro Gonzalez Inarritu, Nicolas Giacobone, Alexander Dinelaris, Armando Bo, "Birdman."
• Original Score: Johann Johannsson, "The Theory of Everything."
• Original Song: "Glory" (music by John Legend, Common), "Selma."
TELEVISION
• Series, Drama: "The Affair."
• Actor, Drama: Kevin Spacey, "House of Cards."
• Actress, Drama: Ruth Wilson, "The Affair."
• Series, Musical or Comedy: "Transparent."
• Actress, Musical or Comedy: Gina Rodriguez, "Jane the Virgin."
• Actor, Musical or Comedy: Jeffrey Tambor, "Transparent."
• Miniseries or Movie: "Fargo."
• Actress, Miniseries or Movie: Maggie Gyllenhaal, "The Honorable Woman."
• Actor, Miniseries or Movie: Billy Bob Thornton, "Fargo."
• Supporting Actress, Series, Miniseries or Movie: Joanne Froggatt, "Downton Abbey.
• Supporting Actor, Series, Miniseries or Movie: Matt Bomer, "The Normal Heart."
• Cecil B. DeMille Lifetime Achievement Award: George Clooney
The Golden Globe Awards screens live from 2pm on Vibe, Sky channel 006.
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