"Hey, @realDonaldTrump, Listen the f**k up," she began.
"I was sexually assaulted twice. Once when I was a teenager. I never filed a police report and it took me 30 years to tell me [sic] parents.
"If any survivor of sexual assault would like to add to this please do so in the replies. #MeToo."
Thousands of women have taken inspiration from Milano and come forward with their stories, prompting a new #MeToo era hashtag — #WhyIDidntReport.
The new hashtag quickly became a trending conversation starter on Twitter in the United States, as people, mostly women, vented outrage over past sexual transgressions and to stand in solidarity with Dr Ford.
Tens of thousands of tweets have since been posted to the social media platform as users explained why they had not reported occasions on which they too had been sexually assaulted in the past.
And Milano wasn't the only star to come forward. Double Jeopardy actress Ashley Judd, who is also one of dozens of women who accused Harvey Weinstein of harassment, tweeted: "The first time it happened, I was 7. I told the first adults I came upon. They said 'Oh, he's a nice old man, that's not what he meant.'
"So when I was raped at 15, I only told my diary. When an adult read it, she accused me of having sex with an adult man."
On Friday (local time), Mr Trump also abandoned any restraint over Dr Ford as he demanded that the Republican-controlled Senate get on with confirming Kavanaugh — and so tilt the highest US judicial body further to the right before November's crucial midterm elections.