However, Adele refused to be deterred by the problems surrounding her and soldiered on through track.
She took to her Twitter account to explain that the piano microphones fell onto the piano strings and knocked the instrument out of tune.
And after leaving the ceremony Adele went to famous LA fast food joint In-N-Out Burger to comfort herself after a challenging evening.
Neil Portnow - the President of the National Academy of Recording Arts and Sciences, the body that organises the Grammys - paid tribute to Adele for unwavering professionalism on stage.
Speaking to UsMagazine.com, he said: "All credit to her for the professional that she is. The show must go on. She did a great job. We certainly don't like it when those things happen, but those things happen sometimes."
Portnow also elaborated on Adele's Twitter post which revealed the tuning issues had been caused by the falling microphone.
He said: "The show has over 20 performances, each performance is a complete set change. We change over the microphones, wires ... My understanding is that, just like this kind of microphone, something like that happened, the microphone dropped into the strings. You've seen the inside of a piano. You could actually hear it in the control room."
Portnow admitted the accident was unfortunate for everyone involved, and especially 27-year-old Adele, but insists mishaps rarely occur at the Grammys.
He said: "These things happen. Rarely on our show, by the way. We have an extraordinary track record ... That being said, when you're performing, particularly an intimate performance like she was doing, she's doing a solo vocal with a piano, it can be unnerving."
Other performances on the night came from Justin Bieber - who brought his little brother to the ceremony with him - The Weeknd and Lady Gaga who gave a tribute to the late David Bowie, backed by Nile Rodgers, who produced the star's album Let's Dance.
- Bang! Showbiz