While Friday was Twitter's second-worst loss since it went public in November 2013, the stock has still doubled in value over the last 12 months.
Long criticized for allowing bad behavior to run rampant on its platform, Twitter has begun to crack down, banning accounts that violate its terms and making others less visible.
Twitter is now attempting to rein in the worst offenders after years as one of the Wild West corners of the internet.
At the same time, it must convince people it's the go-to platform in social media, even though it is dwarfed right now by Facebook.
Facebook has more than 2.23 billion users while its apps WhatsApp, Instagram and Messenger each have over 1 billion.
Twitter on Friday reiterated its efforts to "to invest in improving the health of the public conversation" on its platform, making the "long-term health" of its service a priority over short-term metrics such as user numbers.
As part of these efforts, Twitter said that as of May, its systems identified and challenged more than 9 million accounts per week that are potentially spam or automated, up from 6.4 million in December 2017. The company has previously disclosed these numbers.
A Washington Post report put the total number of suspended accounts in May and June at 70 million. The Associated Press also found that Twitter suspended 56 million such accounts in the last quarter of 2017. While Twitter maintains that most of these accounts were dormant and thus not counted in the monthly user figure, the company also warned that its cleanup efforts could affect its counted user base without giving specific numbers.
"We want people to feel safe freely expressing themselves and have launched new tools to address problem behaviors that distort and distract from the public conversation," CEO Jack Dorsey said in a prepared statement.
Twitter's market value dropped by more than US$6 billion ($8.8b) Friday, to around US$26b. Investors still value Facebook at US$503b. Facebook lost US$119b in value on Thursday.
Twitter's second-quarter net income hit US$100.1 million, after a loss last year during the same period. It's the company's third profit in a row, the third it has ever posted.
Per-share, the San Francisco company's net income was 13 cents, or 17 cents adjusted, in line with expectations, according to a poll by Zacks Investment Research.
Revenue of US$710.5m, up 24 per cent and edging out expectations of US$696m.