Sky chief executive John Fellet said the organisation would have a team monitoring the web and social media for piracy come Saturday night, saying he would rather not have to take legal action, but would if necessary.
The two companies last year successfully filed court proceedings against seven people who illegally streamed Parker's fight against Solomon Haumono, promising at the time to "up the ante" if people ignored the tough line.
Duco previously estimated more than 100,000 people watched illegal streams of Parker's fight against Carlos Takam in May last year.
At a cost of $49.99 pay-per view, the illegal streaming could have cost organisors more than $5 million in lost revenue.
Sky and Duco Events, which are organising Saturday's fight, have come under fire for the $50 pay-per-view price tag to watch the event.
Duco's David Higgins said with no government funding, fans needed to understand the commercial realities.
According to Sky, producing the event would take around 400 people including call centre staff, camera and studio operators, presenters and support staff.
Fellet said it was these people that suffered when people used illegal streaming.
"Piracy or illegal streaming is no different to walking into a store and grabbing a physical item; it still represents the value of a lot of hard work by a lot of people," he said.
"It may be fun, it may be exciting, but at the end of the day you are taking money out of the athletes, the actors and the production people and even people here in this building."
Parker's fight would be available on Fan Pass and online at the pay-per-view price.
