The company told Eater it was the first formal complaint against Batali and that he was reprimanded and required to attend training.
Batali will also take leave from his ABC cooking show, The Chew.
"We have asked Mario Batali to step away from The Chew while we review the allegations that have just recently come to our attention," the network said Monday.
"ABC takes matters like this very seriously as we are committed to a safe work environment. While we are unaware of any type of inappropriate behaviour involving him and anyone affiliated with the show, we will swiftly address any alleged violations of our standards of conduct."
A wave of sexual misconduct allegations have upended the political scene and embroiled Hollywood, gaining momentum after shocking allegations of abuse and assault by Harvey Weinstein.
The #metoo movement has brought down Kevin Spacey, Louis C.K., Matt Lauer, and led to resignations in the US House and Senate. There are new calls for President Donald Trump to address sexual misconduct allegations that he's faced.
Last week Time magazine named the "silence breakers," those that have shared their stories about sexual assault and harassment, as Person of the Year.
The 57-year-old Batali was well known in culinary circles, taking jobs early in his career as a sous chef at the Four Seasons in Santa Barbara and San Francisco.
His career took off after opening Po in New York City in the early 1990s, and he skyrocketed to fame with the airing of Molto Mario, a show that ran on the Food Network for eight years, until 2004. It was there that his signature look, a fleece vest, shorts, and orange Crocs, became instantly recognizable to most people.
The Food Network, which was planning to relaunch Molto Mario, said on Monday that it was placing its plans on hold.
"Food Network takes matters like this very seriously," it said.
Batali also co-owns restaurants in a handful of cities. The Batali & Bastianich Hospitality Group owns or operates several restaurants, including Babbo in New York, Carnevino Italian Steakhouse in Las Vegas and Pizzeria Mozza in Los Angeles.
It's also a partner in Eataly, an Italian food hall and grocer, which has locations in New York, Chicago and Boston.
Batali has long been socially active.
The Mario Batali Foundation advocates child nutrition. He has come out forcefully against hydraulic fracturing, a method used to extract oil and gas.
- AP