Jetstar said around 80 per cent of its flights will operate as scheduled over the weekend but close to 100 flights will be cancelled on Saturday and Sunday.
"Overall, Jetstar will cancel 44 services on Saturday, 14 December and 46 services on Sunday, 15 December, and Jetstar will contact impacted customers from today. On average Jetstar operates about 370 flights a day," Jetstar CEO Gareth Evans said.
"We expect to get 95 per cent of our customers on their way on the same day.
"Any customers travelling from 13 December to 20 December can also request a full refund prior to flying."
Australian Federation of Air Pilots members will walk off the job for four hours on Saturday and Sunday following failed conversations around stalled wage negotiations. It is the first time Jetstar pilots have taken protected industrial action since the airline was founded 15 years ago.
Mr Evans said the strike would have an impact on travellers from Friday; however, the airline would not buckle "at any cost".
"We know the union's actions are creating uncertainty for travellers," he said.
"We remain committed to reaching a new agreement to support the great work our people do every day but not at any cost."
The three days of strikes – which fall less than two weeks out from Christmas – come after the budget airline rejected workers' demands for pay increases, minimum hours on the job and safety improvements.
Transport Workers Union (TWU) leaders have told the company about the planned stoppages after a majority of its members voted to launch protected industrial action.
TWU national secretary Michael Kaine said the airline needed to explain why it had rejected the "modest" requests.
"Jetstar won't listen when these workers say to them that they simply can't survive on 20 hours of work a week … when they make sure Jetstar knows they are on the lowest pay in the industry," Mr Kaine said.
"They are finding it harder and harder to support their families, and Jetstar won't listen when these workers repeatedly say that their jobs are not safe because there are too few workers lugging around thousands and thousands of kilos of baggage and having to service overlapping aircraft."
Crews at Melbourne Airport will strike from 9.30am to 11.30am and again from 4pm to 6pm. In Sydney, staff will walk off the job between 5.30am to 7.30am and 5.30pm to 7.30pm, while in Brisbane and Adelaide there will be a strike from 6am to 8am and 6pm to 8pm.
It is understood passengers impacted by cancellations will be notified by the airline today.
