Qantas will use recycled American cooking oil to help power a biofuel trial flight tomorrow.
The aircraft will use a mix of biofuel and conventional jet fuel for the Sydney-Adelaide return service. Produced by Dutch firm SkyNRG, the fuel has been used by several other airlines.
Qantas says its "life cycle" carbon footprint is around 60 per cent smaller than that of conventional jet fuel. It is part of a long-term plan to reduce a fuel bill that totalled A$3.6 billion last year.
The airline said the goal of the flights was to raise awareness about the potential for sustainable aviation fuel in Australia.
Spokesman Tom Woodward said that while other airlines had run biofuel flights, debate about alternative aviation fuel in Australia had been limited.