Rotoiti will be looking for their third win in a row, including last weekend's 52-27 home victory over Greerton Marist.
Te Puke look to get their first back-to-back wins in the competition after a 25-22 away win over Whakatane Marist last Saturday.
Rotoiti vice-captain Joe Royal said the side were confident of getting a positive result but had to be be wary against tough opposition.
"Te Puke have been a pretty tough side all competition and it's a big game that all of us are excited about," he said.
"But the boys will have to take their confidence from the last two games where they have fought for the win.
"We are not looking too far ahead, if we get over the Te Puke hurdle and see how the other results go then hopefully things will go our way.
"Currently the team's focus is just a one game at a time mentality."
Te Puke coach Craig Jeffries said much was at stake for both sides and was well aware of the Rotorua team's strengths.
"There is a lot riding on it but the big picture doesn't really come into it at the moment as we are just trying to win the next game," he said.
"The boys will lift as home games are always really important to us. We like to protect our patch. We have to win and anything else is basically end of the season for us. We don't want to have to rely on any other team's misfortune to promote us."
"Their forwards are generally really good and they have a couple of big game players in (lock) Baden Wardlaw and (flanker) Zak Hohneck. They are bloody good footballers and they base a lot of their play around those better players.
"So it has to be in the backs for us but their backline is not too shabby either. They are going pretty well and we were lucky to beat them in the first round."
Additional reporting Peter White.
Saturday's games:
Baywide Premier 1
Kick-off 2.45pm/Development kick-off 1pm
Te Puke Sports v Rotoiti, Murray Salt Stadium
Mount Maunganui v Greerton Marist, Blake Park
Rangataua v Whakatane Marist, Te Ariki Park
Tauranga Sports v Te Puna, Tauranga Domain
Baywide Premier 2
Kick-off 2.45pm/Development kick-off 1pm
Ngongotaha v Waikite, Ngongotaha Domain
Paroa v Whakarewarewa, Lawson Park
Arataki v Marist St Michael's, Arataki Park
Poroporo v Opotiki, Poroporo
Baywide Division 1
Kick-off 2.45pm
Papamoa v Katikati, Gordon Spratt Reserve
Ruatoki v Edgecumbe, Tuhoe Park
Kahukura v Rangiuru, Kuirau Park
Te Teko v Judea, Dunderdale Park