The application for Ngongotahā's Special Housing Area, which would have seen up to 80 new homes built, has been declined by the Minister for Housing and Urban Development.
In a statement on the Rotorua Lakes Council website, Rotorua mayor Steve Chadwick described the decision as both frustrating and disappointing.
"It is extremely frustrating the application has been declined. The people of Rotorua are being denied housing at a time when they need it the most.
"We have the second highest rate of registered homelessness in the country and we are bitterly disappointed the Minister declined this application."
Initial plans for the 16ha site, launched in March 2018, included 190 dwellings but an update in June said up to 80 lots would be created. Developer Martin Schilt said the reduction was due to time constraints.