The chairperson of local animal welfare group Watchdog! has likened the conditions at Hastings Pound to a "canine concentration camp".
Struggling with an outbreak of parvovirus, the facility is taking in as few new dogs as possible and maintaining a stringent disinfectant regime to deter further spread.
"I'm of the view we are doing everything we physically can to minimise the disease and make sure we are not taking dogs into the pound unless it is a last resort - if we cannot prove ownership, or if they have been involved in some kind of serious attack," Hastings Mayor Lawrence Yule said.
Due to the incubation period of parvo, which is typically seven to 14 days from exposure to the first signs of disease, it was necessary to continue testing for the rest of the week.
Just yesterday, another puppy was found to have the virus and had to be put to sleep - taking the tally to five.