The speed limit threshold has been lowered to 4km/h over the limit for the long weekend.
Police are warning they will be out in force throughout the region - targeting speeding, seatbelts and drink-driving.
Eastern road policing manager Inspector Matt Broderick said Labour Weekend was traditionally a busy time on local roads due to an influx of visitors.
"Long weekends inevitably see an increase in traffic on our roads and our aim is to make sure the roads are kept safe and drivers are doing their bit. We just want everyone to have an enjoyable weekend and to get to their destinations safely."
One person was killed on the roads during Labour Weekend last year - the lowest number of road deaths recorded during a Labour Weekend since records began.